| Environmental Health Issues Franz Kiefer,1 Olga Cumpelik,1 Rashmeet Reen,1
Johannes Doehmer,2 and Friedrich J. Wiebel1 1Institut für Toxikologie, GSF-Forschungszentrum für
Umwelt und Gesundheit, Neuherberg, Germany; 2Institut für
Toxikologie und Umwelthygiene der Technischen Universität, München,
Germany Abstract
The effect of 2-aminofluorene (2-AF) on the toxicity of 2-aminoanthracene (2-AA) and 1,6-dinitropyrene (1,6-DNP) was studied in N-acetyltransferase-proficient V79-NHr1A2 cells genetically engineered for the expression of cytochrome P4501A2, and in wild-type V79-NH cells. 2-AA inhibited the growth of V79-NHr1A2 cells and induced the formation of micronuclei at concentrations of 0.1 to 1.0 µM, but was virtually without toxic effects at a concentration of 10 µM. Addition of 2-AF protected against the cytotoxic and genotoxic effects elicited by low concentrations of 2-AA. Half-maximum protection was observed at 0.2 to 0.5 µM 2-AF. The arylamine also prevented the cytotoxicity caused by 1,6-DNP in V79-NH cells and completely suppressed the formation of 1-acetylamino-6-nitropyrene from 1,6-DNP in these cells. The results indicate that arylamines and related N-hydroxyarylamines are substrates for the same acetyltransferase in V79-NH cells. In consequence, arylamines are capable of suppressing the activation of their proximate cytotoxic and genotoxic products in these cells and, presumably, in vivo. -- Envrion Health Perspect 102(Suppl 6) :95-97(1994) Key words: acetyltransferase, 2-aminoanthracene, 2-aminofluorene, arylamines, cytotoxicity, genotoxicity, 1, 6-dinitropyrene, micronuclei, V79-NH cells, V79-NHr1A2 cells
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