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Garet P. Lahvis,¹ Randall S. Wells,² Douglas W. Kuehl,³ Jennifer L. Stewart,⁴ Howard L. Rhinehart,⁵ and Charles S. Via⁶

¹Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland; ²Chicago Zoological Society, Sarasota, Florida; ³U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota; ⁴Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland; ⁵Dolphin Biology Research Institute, Sarasota, Florida; ⁶University of Maryland School of Medicine and Research Service, Baltimore Veterans Administration Medical Center, Baltimore, Maryland

Abstract

Since 1987, large-scale mortalities of dolphins have been reported along the Atlantic coast of North America, in the Gulf of Mexico, and in the Mediterranean Sea. Autopsied bottlenose dolphins, Tursiops truncatus, which were collected from the large-scale mortality along the Atlantic coast in 1987 to 1988, exhibited opportunistic infections indicative of immune dysfunction. Further, these animals had high levels of chlorinated hydrocarbons, such as PCBs and DDT, that can suppress immune functions. The purpose of this study was to determine whether there is a relationship between chemical contaminant exposure and immune response in free-ranging dolphins. In June of 1991, peripheral blood was obtained from members of a bottlenose dolphin population that resides along the west coast of Florida. Peripheral blood lymphocyte responses to Concanavalin A (Con A) and phytohemagglutinin (PHA) were determined in vitro and compared by regression analysis with contaminant concentrations in whole blood from a small subset of these animals (n=5) . These data indicate that a reduced immune response in these bottlenose dolphins was correlated with increasing whole blood concentrations of several contaminants. Specifically, inverse correlations were found between Con A-induced lymphocyte proliferation and tetrachlorinated to octachlorinated biphenyls (r² values ranged from 0.70 to 0.87) . Con A-induced lymphocyte responses also correlated inversely with p,p' DDT (r² values of 0.73 and 0.79) ; o,p' - DDE (r² values of 0.93 and 0.96) ; and p,p' - DDE (r² values of 0.73 and 0.81) . -- Environ Health Perspect 103(Suppl 4) :00-00 (1995)

Key words: dolphin, Tursiops truncatus, marine, strandings, immune response, lymphocyte, contaminant, polychlorinated hydrocarbons, PCB (polychlorinated biphenyl) , DDT (1, 1, 1-trichloro-2, 2-bis(p-chlorophenyl) ethane)