Environmental Health Perspectives 105, Supplement 6, December 1997

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Radiation-induced Double-strand Modification in Calf Thymus DNA in the Presence of 1,2-Dihydroxy-9,10-anthraquinone and Its Cu(II) Complex

Saurabh Das, Abhijit Saha, and Parikshit Chandra Mandal

Nuclear Chemistry Division, Saha Institute of Nuclear Physics, Calcutta, India

Abstract
When cells or DNA is exposed to ionizing radiation, the radicals produced in the irradiated sample will modify the base-pair region of the double strands. Effects of 1,2-dihydroxy-9,10-anthraquinone (DHA) and its Cu(II) complex on the radiation-induced modification of double-strands in calf thymus DNA were studied using ethidium bromide as a fluorescent probe. Our results show that the Cu(II)-DHA complex is more efficient in modifying the base-pair region in double-stranded DNA compared to free DHA. -- Environ Health Perspect 105(Suppl 6):1459-1462 (1997)

Key words: DNA double-strand modification, calf-thymus DNA, ethidium bromide, anthracyclines, 1,2-dihydroxy-9,10-anthraquinone, Cu(II)-DHA (copper complex of 1,2-dihydroxy-9,10-anthraquinone)

This paper is based on a presentation at the International Conference on Radiation and Health held 3-7 November 1996 in Beer Sheva, Israel. Abstracts of these papers were previously published in Public Health Reviews 24(3-4):205-431 (1996). Manuscript received at EHP 28 February 1997; accepted 3 July 1997.

S. Das is grateful to CSIR, >Delhi, for financial support (Senior Research Fellowship). The authors express their gratitude to the reviewers for critically reviewing the manuscripts and suggesting improvement.

Address correspondence to Dr. P.C. Mandal, Nuclear Chemistry Division, Saha Institute of Nuclear Physics, 1/AF Bidhan Nagar, Calcutta-700 064, West Bengal, India. Telephone: 91 33 337 5343 49. Fax: 91-33-3374637. E-mail: pcm@hp2.saha.ernet.in

Abbreviations: DHA,1,2-dihydroxy-9,10-anthraquinone; ETB, ethidium bromide.

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