Environmental Health Perspectives 105, Supplement 6, December 1997

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Action of Chronic Irradiation on the Cytogenetic Damage of Human Lymphocyte Culture

Svetlana I. Zaichkina, Gella F. Aptikaeva, Olga M. Rozanova, Asia Kh. Akhmadieva, Elena N. Smirnova, and Elena E. Ganassi

Laboratory of Cytogenetics, Institute of Theoretical and Experimental Biophysics, Pushchino, Moscow Region, Russia

Abstract
The action of chronic irradiation (dose rate 2.9 Gy/day) on human lymphocyte culture was investigated. Whole blood was irradiated at 37°C. Aliquots (0.2 ml) of whole blood were cultivated by the standard method. A medium containing phytohemagglutinin was added immediately after irradiation. All structural chromosome- and chromatid-type changes were recorded. The experimental data showed that the conditions of irradiation of lymphocytes affected neither the background level of chromosome damage nor their radiosensitivity. The obtained dose-response curve of chromosome aberrations was described by a linear regression, which then became a plateau. There is no statistically significant difference between the results for the low doses (10-50 cGy) of chronic and acute radiation. -- Environ Health Perspect 105(Suppl 6):1441-1443 (1997)

Key words: chromosome aberrations, human lymphocytes, chronic gamma-irradiation

This paper is based on a presentation at the International Conference on Radiation and Health held 3-7 November 1996 in Beer Sheva, Israel. Abstracts of these papers were previously published in Public Health Reviews 24(3-4):205-431 (1996). Manuscript received at EHP 12 August 1997; accepted 3 October 1997.

Address correspondence to Dr. S.I. Zaichkina, Laboratory of Cytogenetics, Institute of Theoretical and Experimental Biophysics, Pushchino, Moscow Region, 142292 Russia. Telephone: 7 0967 739349. Fax: 7 0967 790553. E-mail: zaichkina@venus.iteb.serpukhov.su

Abbreviations used: cGy, centigray; Gy, gray.

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