| The Pyrethroid Pesticide Esfenvalerate Suppresses the Afternoon Rise of Luteinizing Hormone and Delays Puberty in Female Rats Michelle D. Pine, Jill K. Hiney, Boyeon Lee, and W. Les Dees Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, USA Abstract
Background: One of the most widely used classes of insecticides is the synthetic pyrethroids. Although pyrethroids are less acutely toxic to humans than to insects, in vitro studies have suggested that pyrethroids may be estrogenic. Objectives: We assessed pubertal effects by orally administering 0.5, 1.0, and 5.0 mg/kg/day of the type II pyrethroid esfenvalerate (ESF) to female rats beginning on postnatal day (PND) 22 until vaginal opening. ESF administration suppresses serum estradiol and delays pubertal onset. Materials and methods: To assess possible hypothalamic and/or pituitary effects, animals received 0.5 or 1.0 mg/kg ESF or corn oil on PNDs 22–29. On PND30, we drew three blood samples (200 µL) from each rat at 15-min intervals beginning at 1000 hours, and again at 1500 hours. To test hypothalamic responsiveness, after the third afternoon sample, all animals received an intravenous injection of N-methyl-d,l-aspartic acid (NMA ; 40 mg/kg) , and then we drew two more samples. We performed a second experiment as above except that animals received luteinizing hormone–releasing hormone (LHRH ; 25 ng/rat) to test pituitary responsiveness. Results: Basal levels of luteinizing hormone (LH) in the afternoon hours were higher in control animals than in animals treated with 1.0 mg/kg ESF (p < 0.05) . Furthermore, NMA- and LHRH-stimulated LH release was similar in control and ESF-treated animals, indicating that both hypothalamic and pituitary responsiveness, respectively, were unaffected. Conclusions: Although the hypothalamus is able to respond to exogenous stimuli, absence of a normal afternoon rise in LH would indicate a hypothalamic deficit in ESF-treated animals. Key words: esfenvalerate, hypothalamus, luteinizing hormone, puberty, pyrethroid pesticides. Environ Health Perspect 116:1243–1247 (2008) . doi:10.1289/ehp.11119 available via http://dx.doi.org/ [Online 13 May 2008]
Address correspondence to M.D. Pine, Department of Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4458 USA. Telephone: (979) 458-0594. Fax: (979) 847-8981. E-mail: mpine@cvm.tamu.edu The authors declare they have no competing financial interests. Received 4 December 2007 ; accepted 9 May 2008.
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